Nutrilite Health Institute, Amway
Purpose: To prepare a new EGCG delivery system by utilizing nano-EGCG loaded thermoreversible in situ hydrogel to enhance EGCG stability and resident time in adipose tissue ablation. The present study may poetically provide a better way to achieve a maximum therapeutically benefit of nano-EGCG on abdominal obesity treatment.
Methods: Nano-EGCG was prepared using a phase inversion-based process by encapsulating EGCG into chitosan coated nanostructured lipid carriers. Nano-EGCG was then dispersed into thermoreversible Pluronic® F127-based hydrogel by cold method. The sol-gel transition temperatures were evaluated using inverted tube method. The stability of native EGCG, nano-EGCG solution, nan-EGCG loaded gel were investigated in 1×PBS (pH=7.4) at 37°C for 15 days. In vitro EGCG release from nano-EGCG loaded in situ gel were detected by a membrane-less diffusion method. In vitro cytotoxicity and cellular triglyceride accumulation in 3T3-L1 adipocytes cells were determined by MTT and an enzymatic method, respectively.