Dr.Maryam Vaezi
Kermanshah University of Medical Sciences, Iran
Biography:
Dr.Maryam Vaezi,Kermanshah University of Medical Sciences, Iran
Abstract:
Abstract
Objective: The aim of this study is to investigate the effect of diazepam on isolated hearts of hypothyroid rats.
Methods: Experimental study was conducted on 4 rat groups. (30; n=7-8) including group I (control), group II which was treated with propylthiouracyl (0.1gr/lit in drinking water for 30 days), group III which was received a daily injection of diazepam 0.5mg/kg for 5 days and group IV which was treated with the same protocol as groups II and III. The isolated hearts passed baseline, ischemia and reperfusion. Left ventricular developed pressure, heart rate and rate pressure product (RPP) were calculated. The released lactate dehydrogenase (LDH) was measured in effluent.
Results: RPP significantly decreased in the group II compared with group I and IV (p=0.001) at baseline. Recovery percentage in reperfusion significantly increased in group II versus I and IV (P=0.009, P=0.002 respectively). The LDH in the group II was significantly lower than that of I and II (P=0.014, P=0.004 respectively).
Conclusion: The cardiac function is affected by hypothyroidism including decreased RPP at baseline and increased recovery percentage in reperfusion. Diazepam eliminated these effects, probably through affecting on mitochondrial peripheral benzodiazepine receptors (PBR) densities and cyclic AMP (cAMP) levels.